Knowles, who was the group’s former manager, claims the incident took place when they toured with Jagged Edge.
Knowles told Vlad TV: ”Now, remember the girls are minors. They’re 16 years old… the guys are 21 and 22 years old. I have a fiduciary duty with minors, by the law, there’s a certain way I have to manage that.
”I got a call from Kelly and Beyoncé… saying that they were constantly being harassed by two of the members in Jagged Edge.
”I couldn’t have that. I literally had to put the guys off the bus in Baton Rouge, in Lousiana. That began all of this drama.”
Beyoncé, Rowland and members of Jagged Edge are yet to address the claims.
Meanwhile, Beyoncé recently addressed speculation that Destiny’s Child were high during a much-shared 2001 interview.
Answering a Q&A in which one fan made reference to the clip, the singer said the interview had taken place “after a 16-hour press day”, adding: “Not marijuana!!”